This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
catalog :
35512
citations: 3
Reference
Asgarihafshejani A, Honoré E, Michon F, Laplante I, Lacaille J. Long-term potentiation at pyramidal cell to somatostatin interneuron synapses controls hippocampal network plasticity and memory. iScience. 2022;25:104259 pubmed publisher
Tasaka G, Guenthner C, Shalev A, Gilday O, Luo L, Mizrahi A. Genetic tagging of active neurons in auditory cortex reveals maternal plasticity of coding ultrasonic vocalizations. Nat Commun. 2018;9:871 pubmed publisher
Mattis J, Tye K, Ferenczi E, Ramakrishnan C, O Shea D, Prakash R, et al. Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 2011;9:159-72 pubmed publisher
product information
Catalog Number :
35512
Product Name :
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
article :
doi10.1038/nmeth.1808
id5139
pubmed_id22179551
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutationAddition of a CaMKIIa promoter and a WPRE
backbone_origin
backbone_size5385
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
growth strain :
AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCaMKIIa
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5AGTCCTGCAGTATTGTGTAT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationE123T and T159C
namehChR2
shRNA_sequence
size1646
species
Chlamydomonas reinhardtii
tags
locationC terminal on insert
tagmCherry
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA