This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
catalog :
35510
citations: 2
Reference
Naffaa M, Khan R, Kuo C, Yin H. Cortical regulation of neurogenesis and cell proliferation in the ventral subventricular zone. Cell Rep. 2023;42:112783 pubmed publisher
Mattis J, Tye K, Ferenczi E, Ramakrishnan C, O Shea D, Prakash R, et al. Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 2011;9:159-72 pubmed publisher
product information
Catalog Number :
35510
Product Name :
pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
article :
doi10.1038/nmeth.1808
id5139
pubmed_id22179551
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutationAddition of an Ef1a promoter, lox sites and WPRE
backbone_originStratagene
backbone_size5605
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
growth strain :
AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5AscI
promoterEf1a
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5CACCCACACAAAGGAAAAGGGCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationE123T and T159C
namehChR2
shRNA_sequence
size1646
species
Chlamydomonas reinhardtii
tags
locationC terminal on insert
tagmCherry
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA