This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-CaMKIIa-hChR2(E123A)-mCherry
catalog :
35506
citations: 4
Reference
Naffaa M, Khan R, Kuo C, Yin H. Cortical regulation of neurogenesis and cell proliferation in the ventral subventricular zone. Cell Rep. 2023;42:112783 pubmed publisher
Nordman J, Li Z. The Dorsal Raphe Regulates the Duration of Attack through the Medial Orbitofrontal Cortex and Medial Amygdala. Eneuro. 2020;7: pubmed publisher
Nordman J, Ma X, Li Z. Traumatic Stress Induces Prolonged Aggression Increase through Synaptic Potentiation in the Medial Amygdala Circuits. Eneuro. 2020;7: pubmed publisher
Mattis J, Tye K, Ferenczi E, Ramakrishnan C, O Shea D, Prakash R, et al. Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 2011;9:159-72 pubmed publisher
product information
Catalog Number :
35506
Product Name :
pAAV-CaMKIIa-hChR2(E123A)-mCherry
article :
doi10.1038/nmeth.1808
id5139
pubmed_id22179551
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutationAddition of a CaMKIIa promoter and a WPRE
backbone_origin
backbone_size5385
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
growth strain :
AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCaMKIIa
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5AGTCCTGCAGTATTGTGTAT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationE123A
namehChR2
shRNA_sequence
size1646
species
Chlamydomonas reinhardtii
tags
locationC terminal on insert
tagmCherry
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA