pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry | Addgene 35502 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

catalog :

35502

citations: 2

Reference
Steude A, Witts E, Miles G, Gather M. Arrays of microscopic organic LEDs for high-resolution optogenetics. Sci Adv. 2016;2:e1600061 pubmed publisher
Yizhar O, Fenno L, Prigge M, Schneider F, Davidson T, O Shea D, et al. Neocortical excitation/inhibition balance in information processing and social dysfunction. Nature. 2011;477:171-8 pubmed publisher

product information

Catalog Number :

35502

Product Name :

pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

article :

doi	10.1038/nature10360
id	5138
pubmed_id	21796121

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV

backbone_mutation

Addition of a CaMKIIa promoter and a WPRE

backbone_origin

backbone_size

5385

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

Other

growth notes :

ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.

growth strain :

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

origin :

pi :

alt_names

SSFO-mCherry

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	CaMKIIa
sequencing_primer_3	GCAATAGCATGATACAAAGG
sequencing_primer_5	AGTCCTGCAGTATTGTGTAT
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

C128S and D156A

name

hChR2(C128S/D156A)-mCherry

shRNA_sequence

size

1646

species

Chlamydomonas reinhardtii