This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry
catalog :
35500
citations: 2
Reference
Wahis J, Baudon A, Althammer F, Kerspern D, Goyon S, Hagiwara D, et al. Astrocytes mediate the effect of oxytocin in the central amygdala on neuronal activity and affective states in rodents. Nat Neurosci. 2021;24:529-541 pubmed publisher
Yizhar O, Fenno L, Prigge M, Schneider F, Davidson T, O Shea D, et al. Neocortical excitation/inhibition balance in information processing and social dysfunction. Nature. 2011;477:171-8 pubmed publisher
product information
Catalog Number :
35500
Product Name :
pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry
article :
doi10.1038/nature10360
id5138
pubmed_id21796121
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutationAddition of a CaMKIIa promoter and a WPRE
backbone_origin
backbone_size5385
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
growth strain :
AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
origin :
37
pi :
alt_names
C1V1 (t/t)
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCaMKIIa
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5AGTCCTGCAGTATTGTGTAT
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
AEL28923.1
mutationE122T and E162T
nameChR1-VChR1 Chimera
shRNA_sequence
size1809
species
100
Synthetic
tags
locationC terminal on insert
tagmCherry
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA