This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
catalog :
35498
citations: 1
Reference
Yizhar O, Fenno L, Prigge M, Schneider F, Davidson T, O Shea D, et al. Neocortical excitation/inhibition balance in information processing and social dysfunction. Nature. 2011;477:171-8 pubmed publisher
product information
Catalog Number :
35498
Product Name :
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
article :
doi10.1038/nature10360
id5138
pubmed_id21796121
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutationAddition of Ef1a promoter, Lox sites and a WPRE
backbone_origin
backbone_size5590
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Other
growth strain :
AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
origin :
37
pi :
alt_names
C1V1 (t/t)
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5AscI
promoterEf1a
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5CACCCACACAAAGGAAAAGGGCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
AEL28923.1
mutationE122T and E162T
nameChR1-VChR1 Chimera
shRNA_sequence
size1809
species
100
Synthetic
tags
locationC terminal on insert
tagmCherry
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA