This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUG2K
catalog :
35493
citations: 2
Reference
Iyer S, Zhang S, Yucel S, Horn H, Smith S, Reinhardt F, et al. Genetically Defined Syngeneic Mouse Models of Ovarian Cancer as Tools for the Discovery of Combination Immunotherapy. Cancer Discov. 2021;11:384-407 pubmed publisher
Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani R. Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Cancer Res. 2012;72:1547-56 pubmed publisher
product information
Catalog Number :
35493
Product Name :
pUG2K
article :
doi10.1158/0008-5472.CAN-11-3222
id5137
pubmed_id22318865
bacterial resistance :
Ampicillin
cloning :
backbonepUltra
backbone_mutation
backbone_originMalcom Moore
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5Xba1
promoterUbiquitin C
sequencing_primer_3
sequencing_primer_5ccactacctgagcacccagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationG12V
nameKRAS(G12V)
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
We inserted PCR amplified constitutively active KRAS(G12V) from the pBabe K-Ras 12V plasmid (Channing Der) into pUltra.
resistance markers :
476
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA