This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-RNF17L
catalog :
35413
citations: 1
| Reference |
|---|
Pan J, Goodheart M, Chuma S, Nakatsuji N, Page D, Wang P. RNF17, a component of the mammalian germ cell nuage, is essential for spermiogenesis. Development. 2005;132:4029-39 pubmed
|
product information
Catalog Number :
35413
Product Name :
pEGFP-RNF17L
article :
| doi | 10.1242/dev.02003 |
| id | 5126 |
| pubmed_id | 16093322 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pEGFP-C1 | |
| backbone_mutation | ||
| backbone_origin | Clontech | |
| backbone_size | 4731 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Based on previously reported Rnf17 cDNA sequences, Rnf17-specific primers for 3'RACE were designed to obtain full-length cDNA sequences for Rnf17s and Rnf17l (Wang et al., 2001; Yin et al., 1999). By using 3'RACE (rapid amplification of cDNA ends), the depositors amplified, subcloned and sequenced Rnf17 cDNA fragments from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences). The composite cDNA sequences were deposited in GenBank under the following accession numbers: Rnf17s, AY854010; Rnf17l, AY854011. Rnf17 long transcript cDNA was cloned in frame with EGFP into pEGFP-C1 with HindIII/SmaI. (Genbank: AY854011)
origin :
37
pi :
|
plasmid copy :
Rnf17 cDNA fragments amplified and subcloned from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences).
resistance markers :
| 1452 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments