pWPI-FLAG-hPRKCI | Addgene 35387 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWPI-FLAG-hPRKCI

catalog :

35387

citations: 3

Reference
Li F, Negi V, Yang P, Lee J, Ma K, Moulik M, et al. TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism. Nucleic Acids Res. 2022;50:12723-12738 pubmed publisher
Chen J, Xu Y, Wu P, Chen X, Weng W, Li D. Transcription Factor FOXO3a Overexpression Inhibits the Progression of Neuroblastoma by Regulating the miR-21/SPRY2/ERK Axis. World Neurosurg. 2022;164:e99-e112 pubmed publisher
Muccioli M, Qaisi D, Herman K, Plageman T. Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition. Dev Biol. 2016;412:32-43 pubmed publisher

product information

Catalog Number :

35387

Product Name :

pWPI-FLAG-hPRKCI

article :

doi
id	5119
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pWPI

backbone_mutation

backbone_origin

Addgene

backbone_size

11076

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES. The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4

origin :

pi :

alt_names

PRKCI

atypical Protein Kinase C

cloning

clone_method	Restriction Enzyme
cloning_site_3	PmeI
cloning_site_5	PmeI
promoter
sequencing_primer_3	AAGAATGTGTCTGACAGCTG
sequencing_primer_5	ACTGGAATCCACCATGGAAC
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	DXS1179E, PKCI, nPKC-iota
gene	PRKCI
id	5584

genbank_ids

BAG70257.1

mutation

name

Protein Kinase C iota

shRNA_sequence

size

1820

species

9606

Homo sapiens