HBM-Luc | Addgene 35155 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HBM-Luc

catalog :

35155

citations: 2

Reference
Hu T, Wu Q, Chong Y, Qin H, Poole C, van Riggelen J, et al. FGFR1 fusion kinase regulation of MYC expression drives development of stem cell leukemia/lymphoma syndrome. Leukemia. 2018;32:2363-2373 pubmed publisher
Facchini L, Chen S, Marhin W, Lear J, Penn L. The Myc negative autoregulation mechanism requires Myc-Max association and involves the c-myc P2 minimal promoter. Mol Cell Biol. 1997;17:100-14 pubmed

product information

Catalog Number :

35155

Product Name :

HBM-Luc

article :

doi
id	5097
pubmed_id	8972190

bacterial resistance :

Ampicillin

cloning :

backbone

pGL2-basic

backbone_mutation

backbone_origin

Promega

backbone_size

5597

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

This plasmid is a c-myc promoter-luciferase reporter construct designed to examine the regulation of transcription in response to conditional and constitutive Myc activation and functions as described in the associated publication. Addgene's sequencing results identified a V13C mutation in exon 1 of c-myc when compared to GenBank reference sequence AAA59886.1

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacI
cloning_site_5	KpnI
promoter	c-myc
sequencing_primer_3	Luc_N_Rev
sequencing_primer_5	EBV-rev
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

Contains 2.5 kb of the c-myc minimal promoter

name

Myc promoter

shRNA_sequence

size

2500

species

9606

Homo sapiens