This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p53-(1-393)-E285V-N268D
catalog :
35088
citations: 1
Reference
Sheng Y, Laister R, Lemak A, Wu B, Tai E, Duan S, et al. Molecular basis of Pirh2-mediated p53 ubiquitylation. Nat Struct Mol Biol. 2008;15:1334-42 pubmed publisher
product information
Catalog Number :
35088
Product Name :
p53-(1-393)-E285V-N268D
article :
doi10.1038/nsmb.1521
id3364
pubmed_id19043414
bacterial resistance :
Ampicillin
cloning :
backbonepET15b
backbone_mutation
backbone_originNovagen
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Protein expression in BL21DE3-pLysS. Induce culture around OD600=0.8 with 0.5mM IPTG, 15 oC overnight.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationContains aa 1-393 - E285V-N268D
namep53
shRNA_sequence
size1179
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
732
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA