This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BRCA1-(1057-1234)
catalog :
35076
citations: 1
Reference
Mark W, Liao J, Lu Y, Ayed A, Laister R, Szymczyna B, et al. Characterization of segments from the central region of BRCA1: an intrinsically disordered scaffold for multiple protein-protein and protein-DNA interactions?. J Mol Biol. 2005;345:275-87 pubmed
product information
Catalog Number :
35076
Product Name :
BRCA1-(1057-1234)
article :
doi10.1016/j.jmb.2004.10.045
id5081
pubmed_id15571721
bacterial resistance :
Ampicillin
cloning :
backbonepET15b
backbone_mutation
backbone_originNovagen
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
geneBRCA1
id672
genbank_ids
mutationContains aa 1057-1234
nameBRCA1
shRNA_sequence
size531
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
732
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA