This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDS271
catalog :
34974
citations: 1
Reference
Strickland D, Lin Y, Wagner E, Hope C, Zayner J, Antoniou C, et al. TULIPs: tunable, light-controlled interacting protein tags for cell biology. Nat Methods. 2012;9:379-84 pubmed publisher
product information
Catalog Number :
34974
Product Name :
pDS271
article :
doi10.1038/nmeth.1904
id5263
pubmed_id22388287
bacterial resistance :
Ampicillin
cloning :
backbonepGREG505
backbone_mutationPTEF-Mid2-GFP-LOVpep-TCYC1 cassette inserted into pGREG505
backbone_originEUROSCARF
backbone_size7747
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5SacI
promoterTEF
sequencing_primer_3M13 fwd
sequencing_primer_5M13 rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR332W, KAI1
geneMID2
id851042
genbank_ids
mutationT406A, T407A, V416I
nameMid2-GFP-LOVpep
shRNA_sequence
size2796
species
4932
Saccharomyces cerevisiae
A. sativa
tags
plasmid copy :
GFP(S65T) CDS and other sequences from pGREG plasmids (EUROSCARF). A. sativa LOV2 CDS from Kevin Gardner, UT Southwestern Medical Center
resistance markers :
1178
tags :
High Copy
terms :
LEU2
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA