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product summary
company name :
Addgene
product type :
cDNA
product name :
Ube1/PET21d
catalog :
34965
citations: 25
Reference
Zhu K, Suskiewicz M, Hlou x161 ek Kasun A, Meudal H, Miko x10d A, Aucagne V, et al. DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on protein substrates. Sci Adv. 2022;8:eadd4253 pubmed publisher
Umano A, Fang K, Qu Z, Scaglione J, Altınok S, Treadway C, et al. The molecular basis of spinocerebellar ataxia type 48 caused by a de novo mutation in the ubiquitin ligase CHIP. J Biol Chem. 2022;298:101899 pubmed publisher
Bagga T, Tulsian N, Mok Y, Kini R, Sivaraman J. Mapping of molecular interactions between human E3 ligase TRIM69 and Dengue virus NS3 protease using hydrogen-deuterium exchange mass spectrometry. Cell Mol Life Sci. 2022;79:233 pubmed publisher
Taniguchi S, Ito Y, Kiritani H, Maruo A, Sakai R, Ono Y, et al. The Ubiquitin Ligase RNF34 Participates in the Peripheral Quality Control of CFTR (RNF34 Role in CFTR PeriQC). Front Mol Biosci. 2022;9:840649 pubmed publisher
Anoh R, Burke K, Schmelyun D, Lombardi P. Generation of Monoubiquitin and K63-Linked Polyubiquitin Chains for Protein Interaction Studies. Methods Mol Biol. 2022;2444:271-282 pubmed publisher
Nakasone M, Majorek K, Gabrielsen M, Sibbet G, Smith B, Huang D. Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. Nat Chem Biol. 2022;18:422-431 pubmed publisher
Ikeda M, Watanabe T, Ito A, Fujimuro M. Herpes simplex virus 1 infection induces ubiquitination of UBE1a. Biochem J. 2021;478:261-279 pubmed publisher
Ikeda M, Ito A, Sekine Y, Fujimuro M. UBE1a Suppresses Herpes Simplex Virus-1 Replication. Viruses. 2020;12: pubmed publisher
Lumpkin R, Ahmad A, Blake R, Condon C, Komives E. The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases. Mol Cell Proteomics. 2020;: pubmed publisher
Estabrooks S, Brodsky J. Ubiquitination of disease-causing CFTR variants in a microsome-based assay. Anal Biochem. 2020;604:113829 pubmed publisher
Sakai R, Fukuda R, Unida S, Aki M, Ono Y, Endo A, et al. The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. J Cell Sci. 2019;132: pubmed publisher
Luong P, Hedl M, Yan J, Zuo T, Fu T, Jiang X, et al. INAVA-ARNO complexes bridge mucosal barrier function with inflammatory signaling. elife. 2018;7: pubmed publisher
Foglizzo M, Middleton A, Burgess A, Crowther J, Dobson R, Murphy J, et al. A bidentate Polycomb Repressive-Deubiquitinase complex is required for efficient activity on nucleosomes. Nat Commun. 2018;9:3932 pubmed publisher
Vaughan R, Dickson B, Whelihan M, Johnstone A, Cornett E, Cheek M, et al. Chromatin structure and its chemical modifications regulate the ubiquitin ligase substrate selectivity of UHRF1. Proc Natl Acad Sci U S A. 2018;115:8775-8780 pubmed publisher
Gladkova C, Maslen S, Skehel J, Komander D. Mechanism of parkin activation by PINK1. Nature. 2018;559:410-414 pubmed publisher
Vaughan R, Dickson B, Cornett E, Harrison J, Kuhlman B, Rothbart S. Comparative biochemical analysis of UHRF proteins reveals molecular mechanisms that uncouple UHRF2 from DNA methylation maintenance. Nucleic Acids Res. 2018;46:4405-4416 pubmed publisher
Okiyoneda T, Veit G, Sakai R, Aki M, Fujihara T, Higashi M, et al. Chaperone-Independent Peripheral Quality Control of CFTR by RFFL E3 Ligase. Dev Cell. 2018;44:694-708.e7 pubmed publisher
Nayak D, Sivaraman J. Structure of LNX1:Ubc13~Ubiquitin Complex Reveals the Role of Additional Motifs for the E3 Ligase Activity of LNX1. J Mol Biol. 2018;430:1173-1188 pubmed publisher
Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, et al. p62 filaments capture and present ubiquitinated cargos for autophagy. EMBO J. 2018;37: pubmed publisher
Gladkova C, Schubert A, Wagstaff J, Pruneda J, Freund S, Komander D. An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1. EMBO J. 2017;36:3555-3572 pubmed publisher
Conte A, Sigismund S. Methods to Investigate EGFR Ubiquitination. Methods Mol Biol. 2017;1652:81-100 pubmed publisher
Adler G, Konrad Z, Zamir L, Mishra A, Raveh D, Bar Zvi D. The Arabidopsis paralogs, PUB46 and PUB48, encoding U-box E3 ubiquitin ligases, are essential for plant response to drought stress. BMC Plant Biol. 2017;17:8 pubmed publisher
Maspero E, Polo S. In Vitro Ubiquitination: Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays. Methods Mol Biol. 2016;1449:153-60 pubmed publisher
Nayak D, Sivaraman J. Structural basis for the indispensable role of a unique zinc finger motif in LNX2 ubiquitination. Oncotarget. 2015;6:34342-57 pubmed publisher
Berndsen C, Wolberger C. A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Anal Biochem. 2011;418:102-10 pubmed publisher
product information
Catalog Number :
34965
Product Name :
Ube1/PET21d
article :
doi10.1016/j.ab.2011.06.034
id5053
pubmed_id21771579
bacterial resistance :
Ampicillin
cloning :
backbonePET21d
backbone_mutation
backbone_originNovagen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
UBA1
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5BglII
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesA1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS
geneUBA1
id7317
genbank_ids
NM_153280
mutation
nameUbe1
shRNA_sequence
size3176
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
583
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA