pTRE_tau-LacZ::tTA-H100Y | Addgene 34927 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTRE_tau-LacZ::tTA-H100Y

catalog :

34927

citations: 1

Reference
Reijmers L, Perkins B, Matsuo N, Mayford M. Localization of a stable neural correlate of associative memory. Science. 2007;317:1230-3 pubmed

product information

Catalog Number :

34927

Product Name :

pTRE_tau-LacZ::tTA-H100Y

article :

doi	10.1126/science.1143839
id	5049
pubmed_id	17761885

bacterial resistance :

Ampicillin

cloning :

backbone

N/A

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Mouse Targeting

growth notes :

Contains a bi-cistronic tetO promoter that drives expression of tau-LacZ, as well as a tTA-H100Y that was made doxycycline insensitive by introduction of a point mutation (H100Y) in the Tet binding domain. Also known as pAuto5. Addgene NGS QC analysis identified a 54 bp deletion in the tau open reading frame, relative to the provided sequence from the depositing laboratory. Based on these NGS results, the tau ORF shows 100% identity to amino acids 1-382 of tau isoform X14 [Bos taurus] XP_024835348.1.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	N/A
cloning_site_5	N/A
promoter	tetO
sequencing_primer_3	LacZ-R
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	tau
gene	MAPT
id	281296

genbank_ids

mutation

name

tau

shRNA_sequence

size

4274

species

9913

Bos taurus