This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYFJ16-LplA(W37V) (for E. Coli expression)
catalog :
34838
citations: 4
Reference
Morana O, Nieto Garai J, Björkholm P, Bernardino de la Serna J, Terrones O, Arboleya A, et al. Identification of a New Cholesterol-Binding Site within the IFN-γ Receptor that is Required for Signal Transduction. Adv Sci (Weinh). 2022;9:e2105170 pubmed publisher
Plaks J, Kaar J. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization. Methods Mol Biol. 2019;2012:279-297 pubmed publisher
Baalmann M, Best M, Wombacher R. Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction. Methods Mol Biol. 2018;1728:365-387 pubmed publisher
Uttamapinant C, White K, Baruah H, Thompson S, Fernandez Suarez M, Puthenveetil S, et al. A fluorophore ligase for site-specific protein labeling inside living cells. Proc Natl Acad Sci U S A. 2010;107:10914-9 pubmed publisher
product information
Catalog Number :
34838
Product Name :
pYFJ16-LplA(W37V) (for E. Coli expression)
article :
doi10.1073/pnas.0914067107
id3530
pubmed_id20534555
bacterial resistance :
Ampicillin
cloning :
backbonepYFJ16
backbone_mutation
backbone_originGift from John Cronan
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
coumarin ligase
coumarin PRIME ligase
cloning
clone_methodRestriction Enzyme
cloning_site_3SphI
cloning_site_5NdeI
promoterT5 promoter
sequencing_primer_3CTTTCTCTTCGATTTGCCGG
sequencing_primer_5GAATTCATTAAAGAGGAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb4386, ECK4378, JW4349, slr, yjjF
genelplA
id944865
genbank_ids
mutationMutated Trp37 to Val
nameLplA W37V
shRNA_sequence
size1014
species
E. Coli
tags
locationN terminal on backbone
tagHis Tag
plasmid copy :
Received original LplA plasmid from John Cronan (currently at UI-UC)
resistance markers :
526
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA