pcDNA3-CD68 promoter/enhancer | Addgene 34837 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-CD68 promoter/enhancer

catalog :

34837

citations: 6

Reference
Hou Y, Shi J, Guo Y, Shi G. Inhibition of angiogenetic macrophages reduces disc degeneration-associated pain. Front Bioeng Biotechnol. 2022;10:962155 pubmed publisher
Zhu L, Qian J, Jiang Y, Yang T, Duan Q, Xiao X. PlGF Reduction Compromises Angiogenesis in Diabetic Foot Disease Through Macrophages. Front Immunol. 2021;12:736153 pubmed publisher
Hou Y, Shi G, Guo Y, Shi J. Epigenetic modulation of macrophage polarization prevents lumbar disc degeneration. Aging (Albany NY). 2020;12:6558-6569 pubmed publisher
Smith R, Nasiri B, Kann J, Yergeau D, Bard J, Swartz D, et al. Endothelialization of arterial vascular grafts by circulating monocytes. Nat Commun. 2020;11:1622 pubmed publisher
Grace P, Strand K, Galer E, Urban D, Wang X, Baratta M, et al. Morphine paradoxically prolongs neuropathic pain in rats by amplifying spinal NLRP3 inflammasome activation. Proc Natl Acad Sci U S A. 2016;113:E3441-50 pubmed publisher
Lang R, Rutschman R, Greaves D, Murray P. Autocrine deactivation of macrophages in transgenic mice constitutively overexpressing IL-10 under control of the human CD68 promoter. J Immunol. 2002;168:3402-11 pubmed

product information

Catalog Number :

34837

Product Name :

pcDNA3-CD68 promoter/enhancer

article :

doi
id	5018
pubmed_id	11907098

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

CMV promoter has been removed

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68. Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	none
cloning_site_5	none
promoter
sequencing_primer_3	BGH_rev
sequencing_primer_5	none
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GP110, LAMP4, SCARD1
gene	CD68
id	968

genbank_ids

mutation

name

CD68 promoter/enhancer -680/+140

shRNA_sequence

size

820

species

9606

Homo sapiens