This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAD48-daf-2 RNAi
catalog :
34834
citations: 7
Reference |
---|
Dillin A, Crawford D, Kenyon C. Timing requirements for insulin/IGF-1 signaling in C. elegans. Science. 2002;298:830-4 pubmed
|
product information
Catalog Number :
34834
Product Name :
pAD48-daf-2 RNAi
article :
doi | 10.1126/science.1074240 |
id | 5015 |
pubmed_id | 12399591 |
bacterial resistance :
Ampicillin
cloning :
backbone | pBluescript | ||
backbone_mutation | pBluescript was converted into pAD12, with the introduction of T7 promoters at both sides of MCS. | ||
backbone_origin | Stratagene | ||
backbone_size | 2961 | ||
promoter | |||
sequencing_primer_3 | |||
sequencing_primer_5 | |||
vector_types |
|
growth notes :
Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
origin :
37
pi :
|
plasmid copy :
Andrew Dillin and Cynthia Kenyon, UCSF.
resistance markers :
1146 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments