This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAD43-daf-16 RNAi
catalog :
34833
citations: 4
Reference
Matilainen O, Ribeiro A, Verbeeren J, Cetinbaş M, Sood H, Sadreyev R, et al. Loss of muscleblind splicing factor shortens Caenorhabditis elegans lifespan by reducing the activity of p38 MAPK/PMK-1 and transcription factors ATF-7 and Nrf/SKN-1. Genetics. 2021;219: pubmed publisher
Moll L, Roitenberg N, Bejerano Sagie M, Boocholez H, Carvalhal Marques F, Volovik Y, et al. The insulin/IGF signaling cascade modulates SUMOylation to regulate aging and proteostasis in Caenorhabditis elegans. elife. 2018;7: pubmed publisher
Kitaoka S, Morielli A, Zhao F. FGT-1-mediated glucose uptake is defective in insulin/IGF-like signaling mutants in Caenorhabditis elegans. FEBS Open Bio. 2016;6:576-85 pubmed publisher
Dillin A, Crawford D, Kenyon C. Timing requirements for insulin/IGF-1 signaling in C. elegans. Science. 2002;298:830-4 pubmed
product information
Catalog Number :
34833
Product Name :
pAD43-daf-16 RNAi
article :
doi10.1126/science.1074240
id5015
pubmed_id12399591
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript
backbone_mutationpBluescript was converted into pAD12, with the introduction of T7 promoter at both sides of MCS.
backbone_originStratagene
backbone_size2961
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
RNAi
growth notes :
Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3None
cloning_site_5None
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F(-20)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_R13H8.1
genedaf-16
id172981
genbank_ids
mutation
namedaf-16
shRNA_sequence
size1600
species
6239
Caenorhabditis elegans
tags
plasmid copy :
Andrew Dillin and Cynthia Kenyon, UCSF.
resistance markers :
1146
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA