This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Human_TFAM_NoMTS_pET28
catalog :
34705
citations: 4
Reference
Mohammed I, Schmitz K, Schenck N, Balasopoulos D, Topitsch A, Maier T, et al. Catalytic cycling of human mitochondrial Lon protease. Structure. 2022;30:1254-1268.e7 pubmed publisher
Bürger M, Kaelin S, Leroux J. The TFAMoplex-Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent. Adv Sci (Weinh). 2022;9:e2104987 pubmed publisher
Feric M, Demarest T, Tian J, Croteau D, Bohr V, Misteli T. Self-assembly of multi-component mitochondrial nucleoids via phase separation. EMBO J. 2021;40:e107165 pubmed publisher
Ngo H, Kaiser J, Chan D. The mitochondrial transcription and packaging factor Tfam imposes a U-turn on mitochondrial DNA. Nat Struct Mol Biol. 2011;18:1290-6 pubmed publisher
product information
Catalog Number :
34705
Product Name :
Human_TFAM_NoMTS_pET28
article :
doi10.1038/nsmb.2159
id5006
pubmed_id22037171
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3
geneTFAM
id7019
genbank_ids
mutationEncodes residues 43 246, corresponding to full-length TFAM after cleavage of the N-terminal mitochondrial leader sequence (residues 1 42)
nameTFAM
shRNA_sequence
size612
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6XHis
resistance markers :
665
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA