This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5-TGF beta type I receptor (mL45)
catalog :
34701
citations: 1
Reference
Yu L, Hébert M, Zhang Y. TGF-beta receptor-activated p38 MAP kinase mediates Smad-independent TGF-beta responses. EMBO J. 2002;21:3749-59 pubmed
product information
Catalog Number :
34701
Product Name :
pRK5-TGF beta type I receptor (mL45)
article :
doi10.1093/emboj/cdf366
id5004
pubmed_id12110587
bacterial resistance :
Ampicillin
cloning :
backbonepRK5 modified with flag
backbone_mutationFlag tag with stop codon at the end is inserted between SalI and HindIII.
backbone_originGenentech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
3 amino acids, GSM, were introduced after aa148 for cloning purposes.
origin :
37
pi :
alt_names
TGFb type I receptor
Tgfbr1
R4mL45
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAlk5, Skr4, Tgfr-1, tbetaR-I
geneTgfbr1
id29591
genbank_ids
mutationN265A, D267A, N268A
nameTGF beta type I receptor
shRNA_sequence
size1506
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagFlag
plasmid copy :
This plasmid was derived from pRK5-TbRI-FLAG, Addgene plasmid #14831.
resistance markers :
611
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA