This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP
catalog :
34631
citations: 3
Reference
Weisgerber A, Otruba Z, Knowles M. Syntaxin clusters and cholesterol affect the mobility of Syntaxin1a. Biophys J. 2024;: pubmed publisher
Karasmanis E, Phan C, Angelis D, Kesisova I, Hoogenraad C, McKenney R, et al. Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin. Dev Cell. 2018;46:204-218.e7 pubmed publisher
Knowles M, Barg S, Wan L, Midorikawa M, Chen X, Almers W. Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers. Proc Natl Acad Sci U S A. 2010;107:20810-5 pubmed publisher
product information
Catalog Number :
34631
Product Name :
peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP
article :
doi10.1073/pnas.1014840107
id4974
pubmed_id21076040
bacterial resistance :
Kanamycin
cloning :
backbonepeGFP(N1)
backbone_mutationCMV promoter is partially deleted.
backbone_originclontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
rSYX1a
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NheI
promotercmv
sequencing_primer_3
sequencing_primer_5tgggaggtctatataagcag
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneStx1a
id116470
genbank_ids
NM_053788
mutation
namerat Syntaxin1a
shRNA_sequence
size863
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagmeGFP
plasmid copy :
Delta CMV promoter was cut out from Delta CMV-GFP-Vinculin (waterman lab) with Ase1 and Nhe1to replace CMV promoter in syntaxin 1a GFP in same sites
resistance markers :
1139
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA