This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKD-SepRS-EFSep
catalog :
34623
citations: 1
Reference
Park H, Hohn M, Umehara T, Guo L, Osborne E, Benner J, et al. Expanding the genetic code of Escherichia coli with phosphoserine. Science. 2011;333:1151-4 pubmed publisher
product information
Catalog Number :
34623
Product Name :
pKD-SepRS-EFSep
article :
doi10.1126/science.1207203
id4968
pubmed_id21868676
bacterial resistance :
Kanamycin
cloning :
backbonepKK223-3
backbone_mutationpKD is derived from pKK223-3 (Pharmacia). The ampicillin (Amp) resistance gene was replaced with a kanamycin (Kan) resistance gene. The original multiple cloning site (MCS; NcoI-EcoRI-SacI) was modified by adding an additional ribosome binding site (RBS) and a second MCS (NdeI-BamHI-SalI-HindIII), thus enabling simultaneous protein expression from two genes, both under the control of the same tac promoter.
backbone_originPharmacia
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10 serB - Addgene #34928) and BL21 (BL21 serB - Addgene #34929). These strains are required hosts when using this plasmid. Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
origin :
30
pi :
alt_names
sepS
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5NcoI
promoterPtac
sequencing_primer_3
sequencing_primer_5M13_pUC-Rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMMP0688
geneMMP0688
id2762711
genbank_ids
mutation
nameSepRS
shRNA_sequence
size1613
species
Methanocaldococcuc maripaludis
tags
alt_names
tufB
EF-Tu
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterPtac (from SepRS- operon like)
sequencing_primer_3pBAD_rev
sequencing_primer_5pJR_F2
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB21_03809
genetufB
id8116098
genbank_ids
mutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe219 to Tyr, Thr229 to Ser, and Asn274 to Trp
nameEF-Sep
shRNA_sequence
size1184
species
E. coli
tags
resistance markers :
1137
1198
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA