This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LAMP1-mRFP-FLAG
catalog :
34611
citations: 18
Reference
Omrane M, Ben M barek K, Santinho A, Nguyen N, Nag S, Melia T, et al. LC3B is lipidated to large lipid droplets during prolonged starvation for noncanonical autophagy. Dev Cell. 2023;58:1266-1281.e7 pubmed publisher
Liu K, Kong L, Graham D, Carey K, Xavier R. SAC1 regulates autophagosomal phosphatidylinositol-4-phosphate for xenophagy-directed bacterial clearance. Cell Rep. 2021;36:109434 pubmed publisher
Pero M, Meregalli C, Qu X, Shin G, Kumar A, Shorey M, et al. Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Almacellas E, Pelletier J, Day C, Ambrosio S, Tauler A, Mauvezin C. Lysosomal degradation ensures accurate chromosomal segregation to prevent chromosomal instability. Autophagy. 2020;:1-18 pubmed publisher
Morel M, Shah K, Long W. The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7. J Biol Chem. 2020;295:7970-7980 pubmed publisher
Chen Q, Lei J, Bao J, Wang H, Hao W, Li L, et al. BRCA1 Deficiency Impairs Mitophagy and Promotes Inflammasome Activation and Mammary Tumor Metastasis. Adv Sci (Weinh). 2020;7:1903616 pubmed publisher
Beaumatin F, O Prey J, Barthet V, Zunino B, Parvy J, Bachmann A, et al. mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux. Mol Cell. 2019;76:163-176.e8 pubmed publisher
Latifkar A, Ling L, Hingorani A, Johansen E, Clement A, Zhang X, et al. Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity. Dev Cell. 2019;49:393-408.e7 pubmed publisher
Zogzas C, Mukhopadhyay S. Putative metal binding site in the transmembrane domain of the manganese transporter SLC30A10 is different from that of related zinc transporters. Metallomics. 2018;10:1053-1064 pubmed publisher
Nwadike C, Williamson L, Gallagher L, Guan J, Chan E. AMPK Inhibits ULK1-Dependent Autophagosome Formation and Lysosomal Acidification via Distinct Mechanisms. Mol Cell Biol. 2018;38: pubmed publisher
Kuzu O, Gowda R, Noory M, Robertson G. Modulating cancer cell survival by targeting intracellular cholesterol transport. Br J Cancer. 2017;117:513-524 pubmed publisher
Lampada A, O Prey J, Szabadkai G, Ryan K, Hochhauser D, Salomoni P. mTORC1-independent autophagy regulates receptor tyrosine kinase phosphorylation in colorectal cancer cells via an mTORC2-mediated mechanism. Cell Death Differ. 2017;24:1045-1062 pubmed publisher
Serrano D, Ghobadi F, Boulay G, Ilangumaran S, Lavoie C, Ramanathan S. GTPase of the Immune-Associated Nucleotide Protein 5 Regulates the Lysosomal Calcium Compartment in T Lymphocytes. Front Immunol. 2017;8:94 pubmed publisher
Matsumoto A, Pasut A, Matsumoto M, Yamashita R, Fung J, Monteleone E, et al. mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide. Nature. 2017;541:228-232 pubmed publisher
Ullio C, Brunk U, Urani C, Melchioretto P, Bonelli G, Baccino F, et al. Autophagy of metallothioneins prevents TNF-induced oxidative stress and toxicity in hepatoma cells. Autophagy. 2015;11:2184-98 pubmed publisher
Tang E, Wang C. Single amino acid change in STING leads to constitutive active signaling. PLoS ONE. 2015;10:e0120090 pubmed publisher
Nunes P, Ernandez T, Roth I, Qiao X, Strebel D, Bouley R, et al. Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy. 2013;9:550-67 pubmed publisher
Zoncu R, Bar Peled L, Efeyan A, Wang S, Sancak Y, Sabatini D. mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. Science. 2011;334:678-83 pubmed publisher
product information
Catalog Number :
34611
Product Name :
LAMP1-mRFP-FLAG
article :
doi10.1126/science.1207056
id4958
pubmed_id22053050
bacterial resistance :
Ampicillin
cloning :
backbonepLJM1
backbone_mutation
backbone_origin
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NheI
promoter
sequencing_primer_3pLJM1-3 (5'-GACGTGAAGAATGTGCGAGA-3')
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLGP120
geneLamp1
id25328
genbank_ids
NM_012857.2
mutation
nameLAMP1
shRNA_sequence
size1253
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagmRFP
locationC terminal on insert
tag2xFlag
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA