pLJM60 | Addgene 34610 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLJM60

catalog :

34610

citations: 3

Reference
Csukasi F, Bosakova M, Bárta T, Martin J, Arcedo J, Barad M, et al. Skeletal diseases caused by mutations in PTH1R show aberrant differentiation of skeletal progenitors due to dysregulation of DEPTOR. Front Cell Dev Biol. 2022;10:963389 pubmed publisher
Jabłońska E, Białopiotrowicz E, Szydłowski M, Prochorec Sobieszek M, Juszczyński P, Szumera Ciećkiewicz A. DEPTOR is a microRNA-155 target regulating migration and cytokine production in diffuse large B-cell lymphoma cells. Exp Hematol. 2020;: pubmed publisher
de Moura R, Nascimento L, Ignacio Souza L, Morari J, Razolli D, Solon C, et al. Hypothalamic stearoyl-CoA desaturase-2 (SCD2) controls whole-body energy expenditure. Int J Obes (Lond). 2016;40:471-8 pubmed publisher

product information

Catalog Number :

34610

Product Name :

pLJM60

article :

doi
id	4957
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pLJM1-Flag

backbone_mutation

Made by cloning Age1- FLAG-Sal-gene-Not1-ExcoR1 into the Age1/Ecor1 sites in pLJM1

backbone_origin

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

If you plan on using this plasmid for the vector, know that it has a CMV promoter, a FLAG tag and then genes cloned into Sal/Not sites in the vector. Region of interest: Age(site destroyed)-FLAG-Sal-Gene-Not-EcoR1

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter	CMV
sequencing_primer_3	need custom
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DEP.6, DEPDC6
gene	DEPTOR
id	64798

genbank_ids

mutation

name

deptor

shRNA_sequence

size

species

9606

Homo sapiens