This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CaRQ
catalog :
34558
citations: 1
Reference
Mills E, Truong K. Ca2+-mediated synthetic biosystems offer protein design versatility, signal specificity, and pathway rewiring. Chem Biol. 2011;18:1611-9 pubmed publisher
product information
Catalog Number :
34558
Product Name :
CaRQ
article :
doi10.1016/j.chembiol.2011.09.014
id5321
pubmed_id22195563
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx-3
backbone_mutation
backbone_originPMID: 12864922
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth notes :
MLCK peptide - KRRWKKNFIAVSAANRFKKI IQ peptide - GSAITVQRYVRGIQARAYARFL
origin :
37
pi :
alt_names
a Ca2+-activated CaM-RhoA chimera
CaM-MLCK- RhoA(Q63L)/IQp/RhoA
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoterCMV, T7
sequencing_primer_3
sequencing_primer_5CMV-F, T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
mutationQ63L in RhoA
nameCaRQ
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
locationC terminal on insert
tagYFP
resistance markers :
718
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA