CMV566 empty vector | Addgene 33349 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CMV566 empty vector

catalog :

33349

citations: 1

Reference
Rishi V, Gal J, Krylov D, Fridriksson J, Boysen M, Mandrup S, et al. SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative. J Biol Chem. 2004;279:11863-74 pubmed

product information

Catalog Number :

33349

Product Name :

CMV566 empty vector

article :

doi	10.1074/jbc.M308000200
id	4920
pubmed_id	14702347

bacterial resistance :

Ampicillin

cloning :

backbone

pRc/CMV

backbone_mutation

backbone_origin

5500

backbone_size

promoter

CMV

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

(underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5 -CTAGAAGCTTCATCACATATGGGCATAATCGGGCACGTCGTAGGGATACGCCATGGTGG-3 (underlined bases are restriction site for XbaI).
-AGCTCCACCATGGCGTATCCCTACGACGTGCCCGATTA
TGCCCATATGTGATGAAGCTT-3
The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with NdeI and HindIII, and the following annealed oligonucleotide was inserted with a hemagglutinin epitope (YPYDVPDYASL) and NdeI-HindIII cloning site was made using the following pair of complementary oligonucleotides: 5

origin :

resistance markers :

1114

tags :

High Copy