CMV500 empty vector | Addgene 33348 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CMV500 empty vector

catalog :

33348

citations: 2

Reference
Murillo Garzón V, Gorroño Etxebarria I, Akerfelt M, Puustinen M, Sistonen L, Nees M, et al. Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer. Nat Commun. 2018;9:1747 pubmed publisher
Rishi V, Gal J, Krylov D, Fridriksson J, Boysen M, Mandrup S, et al. SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative. J Biol Chem. 2004;279:11863-74 pubmed

product information

Catalog Number :

33348

Product Name :

CMV500 empty vector

article :

doi	10.1074/jbc.M308000200
id	4920
pubmed_id	14702347

bacterial resistance :

Ampicillin

cloning :

backbone

Rc/CMV

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

CMV

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

(underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5 -CTAGAAGCTTCATCACATATGCTTGTCATCGTCGTCCTTGTAGTCCATGGTGG-3 (underlined bases are restriction site for XbaI). The HindIII restriction site was not regenerated and was reintroduced downstream of the FLAG sequence (DYKDDDDK).
-AGCTCCACCATGGACTACAAGGACGACGATGACAAGCA
TATGTGATGAAGCTT-3
The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with HindIII and XbaI, and the following annealed oligonucleotide was inserted: 5

origin :

resistance markers :

1114

tags :

High Copy