pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha | Addgene 33330 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

catalog :

33330

citations: 1

Reference
Green M, Anderson K, Means A. Characterization of the CaMKK?-AMPK signaling complex. Cell Signal. 2011;23:2005-12 pubmed publisher

product information

Catalog Number :

33330

Product Name :

pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

article :

doi	10.1016/j.cellsig.2011.07.014
id	4910
pubmed_id	21807092

bacterial resistance :

Ampicillin

cloning :

backbone

pSG5

backbone_mutation

backbone_origin

Stratagene

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The S3V, G96R, and P108L mutations were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

origin :

pi :

alt_names

CaMKKalpha

CaMKK1

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter	SV40
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Camkk1
id	60341

genbank_ids

NP_113850

mutation

CaMKKbeta N-terminus (1-164) was fused to body for CaMKKalpha (121-505) using PCR based method; S3V, G96R, and P108L also present.

name

Calcium/Calmodulin dependent protein kinase kinase alpha

shRNA_sequence

size

1623

species

10116

Rattus norvegicus