pSG5-FLAG-CaMKKbeta rat 1-460 | Addgene 33324 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSG5-FLAG-CaMKKbeta rat 1-460

catalog :

33324

citations: 2

Reference
Hall D, Cost N, Hegde S, Kellner E, Mikhaylova O, Stratton Y, et al. TRPM3 and miR-204 establish a regulatory circuit that controls oncogenic autophagy in clear cell renal cell carcinoma. Cancer Cell. 2014;26:738-53 pubmed publisher
Green M, Anderson K, Means A. Characterization of the CaMKK?-AMPK signaling complex. Cell Signal. 2011;23:2005-12 pubmed publisher

product information

Catalog Number :

33324

Product Name :

pSG5-FLAG-CaMKKbeta rat 1-460

article :

doi	10.1016/j.cellsig.2011.07.014
id	4910
pubmed_id	21807092

bacterial resistance :

Ampicillin

cloning :

backbone

pSG5

backbone_mutation

Added blunt end cloning site which was lost during cloning.

backbone_origin

Stratagene

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The truncation was made by placing a stop codon at 460, but sequence was not actually removed. The S3V, G96R, and P108L mutations were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

origin :

pi :

alt_names

CaMKKbeta

CaMKK2

cloning

clone_method	Restriction Enzyme
cloning_site_3	None
cloning_site_5	None
promoter	SV40
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases
gene	Camkk2
id	83506

genbank_ids

NM_031338

mutation

truncated at 460; S3V, G96R, and P108L also present

name

Calcium/Calmodulin dependent protein kinase kinase beta

shRNA_sequence

size

1700

species

10116

Rattus norvegicus