pCasper_Pdf-LexA::VP16 | Addgene 33259 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCasper_Pdf-LexA::VP16

catalog :

33259

citations: 1

Reference
Shang Y, Griffith L, Rosbash M. Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Proc Natl Acad Sci U S A. 2008;105:19587-94 pubmed publisher

product information

Catalog Number :

33259

Product Name :

pCasper_Pdf-LexA::VP16

article :

doi	10.1073/pnas.0809577105
id	4896
pubmed_id	19060186

bacterial resistance :

Ampicillin

cloning :

backbone

pCasPeR3

backbone_mutation

The 2.4-kb Pdf promoter (Stoleru et al., 2004) was cloned into pCasPeR3 between BamHI and BglII sites.

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NotI
promoter	Pdf
sequencing_primer_3	SV40 polyA reverse
sequencing_primer_5	none
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	b4043, ECK4035, exrA, spr, tsl, umuA
gene	lexA
id	948544

genbank_ids

mutation

name

LexA::VP16-SV40 3'UTR

shRNA_sequence

size

species

E. coli