This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
catalog :
33255
citations: 1
Reference
Ilic N, Utermark T, Widlund H, Roberts T. PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Proc Natl Acad Sci U S A. 2011;108:E699-708 pubmed publisher
product information
Catalog Number :
33255
Product Name :
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
article :
doi10.1073/pnas.1108237108
id4894
pubmed_id21876152
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro
backbone_mutation
backbone_origin
backbone_size5168
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Note from the depositing lab: This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSV40
sequencing_primer_3
sequencing_primer_5CTTTATCCAGCCCTCAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationchanged histidine 1047 to arginine and phenylalanine 486 to serine; deleted amino acids 343-346
namePIK3CA
shRNA_sequence
size3200
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHemagglutinin (HA)
plasmid copy :
from JJ Zhao, DFCI, Boston MA.
resistance markers :
419
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA