This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAG250
catalog :
33204
citations: 1
Reference
Garcia D, Baek D, Shin C, Bell G, Grimson A, Bartel D. Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Nat Struct Mol Biol. 2011;18:1139-46 pubmed publisher
product information
Catalog Number :
33204
Product Name :
pAG250
article :
doi10.1038/nsmb.2115
id4883
pubmed_id21909094
bacterial resistance :
Ampicillin
cloning :
backbonepIS2
backbone_mutation
backbone_originAddgene plasmid #12177
backbone_size3745
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5SacI
promoterSV40 early enhancer/promoter
sequencing_primer_3EBV Reverse
sequencing_primer_5Rluc-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSID6-1512
geneNEK6
id10783
genbank_ids
NM_001145001.2
mutation
nameNEK6 3'UTR
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagLuciferase
resistance markers :
21
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA