This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLG-deltaA
catalog :
33151
citations: 1
Reference
Legrain P, Rosbash M. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Cell. 1989;57:573-83 pubmed
product information
Catalog Number :
33151
Product Name :
pLG-deltaA
article :
doi10.1016/0092-8674(89)90127-X
id4878
pubmed_id2655924
bacterial resistance :
Ampicillin
cloning :
backbonepLGSD5
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Modified intron sequence: GACCGTGTTTTTGATATCTATACTAACAGGCCTTTTAATAG
GTATGTTAATATGGTTAACGTCGACCGTGTTTTTGATAT
CTATACTAACAGGCCTTTTAATAG
Original intron sequence:
inserts :
TG1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3LacZ-R
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML024W, RP51A, RPL51A
geneRPS17A
id854984
genbank_ids
mutationThe RP51A sequence was modified by digestion with StyI and HincII, filled in and ligated to delete ~27bp including the 5' splice site
nameRP51A-synthetic minimal intron
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagLacZ
resistance markers :
1102
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA