pAC-luc-TimRA | Addgene 33133 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAC-luc-TimRA

catalog :

33133

citations: 1

Reference
Kadener S, Menet J, Sugino K, Horwich M, Weissbein U, Nawathean P, et al. A role for microRNAs in the Drosophila circadian clock. Genes Dev. 2009;23:2179-91 pubmed publisher

product information

Catalog Number :

33133

Product Name :

pAC-luc-TimRA

article :

doi	10.1101/gad.1819509
id	4873
pubmed_id	19696147

bacterial resistance :

Ampicillin

cloning :

backbone

pAc5.1/V5-His B

backbone_mutation

pAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

Luciferase

growth notes :

pAc-Luc-TimRA was constructed by amplifying the genomic region containing the clk 3 UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	none
cloning_site_5	none
promoter	Actin 5C
sequencing_primer_3
sequencing_primer_5	AC5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG3234, CG3234, Dmel CG3234, Ritsu, TIM, Tim, Tim-1, dTIM, dTim, dtim, mel_tim, rit, s-tim, tim1
gene	tim
id	33571

genbank_ids

mutation

name

Tim-RA 3' UTR

shRNA_sequence

size

species

7227

Drosophila melanogaster