This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRS314-T7-GFP
catalog :
33130
citations: 1
Reference
Dower K, Rosbash M. T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. RNA. 2002;8:686-97 pubmed
product information
Catalog Number :
33130
Product Name :
pRS314-T7-GFP
article :
doi10.1017/S1355838202024068
id4871
pubmed_id12022234
bacterial resistance :
Ampicillin
cloning :
backbonepRS314
backbone_mutationNotI - T7 promoter - TDH3 5'UTR - BamHI - GFP - SalI - TDH3 3' UTR and polyadenylation signal - EcoRI
backbone_originATCC
backbone_size4783
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
T7 promoter driven GFP with the TDH3 5' and 3' flanking sequences, and the TDH3 polyadenylation signal all cloned into pRS314 using NotI and EcoRI.
inserts :
TG1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterT7
sequencing_primer_3
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasespCmGFP_001
genegfp
id7011691
genbank_ids
mutation
nameGFP with TDH3 5' and 3' UTR
shRNA_sequence
size
species
tags
resistance markers :
1102
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA