pACAGW-H2B-PAGFP-AAV | Addgene 33000 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pACAGW-H2B-PAGFP-AAV

catalog :

33000

citations: 3

Reference
Barz C, Garderes P, Ganea D, Reischauer S, Feldmeyer D, Haiss F. Functional and Structural Properties of Highly Responsive Somatosensory Neurons in Mouse Barrel Cortex. Cereb Cortex. 2021;: pubmed publisher
Francis A, Marin M, Singh P, Achuthan V, Prellberg M, Palermino Rowland K, et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun. 2020;11:3505 pubmed publisher
Lien A, Scanziani M. In vivo Labeling of Constellations of Functionally Identified Neurons for Targeted in vitro Recordings. Front Neural Circuits. 2011;5:16 pubmed publisher

product information

Catalog Number :

33000

Product Name :

pACAGW-H2B-PAGFP-AAV

article :

doi	10.3389/fncir.2011.00016
id	4971
pubmed_id	22144948

bacterial resistance :

Ampicillin

cloning :

backbone

N/A

backbone_mutation

backbone_origin

backbone_size

4771

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

Other

adeno associated virus

growth notes :

Plasmid containing the H2B-PAGFP fusion gene was a gift from Andr Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071). Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., M ller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34. There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.

growth temp :

Grow on Stbl2 cells

origin :

pi :

alt_names

histone2b-photoactivatable gfp

cloning

entrez_gene

aliases	GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
gene	H2BC21
id	8349

genbank_ids

mutation

name

H2B-PAGFP

shRNA_sequence

size

1116

species

9606

Homo sapiens

Aequorea victoria