pCTX-myc-HIPK2 KD | Addgene 32997 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCTX-myc-HIPK2 KD

catalog :

32997

citations: 1

Reference
Hikasa H, Ezan J, Itoh K, Li X, Klymkowsky M, Sokol S. Regulation of TCF3 by Wnt-dependent phosphorylation during vertebrate axis specification. Dev Cell. 2010;19:521-32 pubmed publisher

product information

Catalog Number :

32997

Product Name :

pCTX-myc-HIPK2 KD

article :

doi	10.1016/j.devcel.2010.09.005
id	4851
pubmed_id	20951344

bacterial resistance :

Ampicillin

cloning :

backbone	pCTX-myc
backbone_mutation
backbone_origin	Sergei Sokol
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Note from depositing lab: The X. laevis catalytic K217 residue corresponds to the mouse catalytic residue K221, in which a similar mutation, K221A, has been previously investigated.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	EcoRI
promoter	SCMV
sequencing_primer_3	SP6; xHIPK2-R (ttaatgccggtctcgttttc)
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	hipk2
id	495265

genbank_ids

mutation

K217>R (in the catalytic lysine) and STY348-350>AAF (in the activation loop)

name

HIPK2

shRNA_sequence

size

species

8355

Xenopus laevis