This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
3XMEF2-luc
catalog :
32967
citations: 12
Reference
Kim Y, Oh J, Jung S, Kim C, Choi J, Jeon Y, et al. The transcription factor Mef2d regulates B:T synapse-dependent GC-TFH differentiation and IL-21-mediated humoral immunity. Sci Immunol. 2023;8:eadf2248 pubmed publisher
Ortega Muelas M, Roche O, Fernandez Aroca D, Encinar J, Albandea Rodríguez D, Arconada Luque E, et al. ERK5 signalling pathway is a novel target of sorafenib: Implication in EGF biology. J Cell Mol Med. 2021;25:10591-10603 pubmed publisher
Neyroud D, Nosacka R, Callaway C, Trevino J, Hu H, Judge S, et al. FoxP1 is a transcriptional repressor associated with cancer cachexia that induces skeletal muscle wasting and weakness. J Cachexia Sarcopenia Muscle. 2021;: pubmed publisher
Niemann B, Li L, Siegler D, Siegler B, Knapp F, Hanna J, et al. CTRP9 Mediates Protective Effects in Cardiomyocytes via AMPK- and Adiponectin Receptor-Mediated Induction of Anti-Oxidant Response. Cells. 2020;9: pubmed publisher
Mukai J, Cannavó E, Crabtree G, Sun Z, Diamantopoulou A, Thakur P, et al. Recapitulation and Reversal of Schizophrenia-Related Phenotypes in Setd1a-Deficient Mice. Neuron. 2019;104:471-487.e12 pubmed publisher
Wang C, Arrington J, Ratliff A, Chen J, Horton H, Nie Y, et al. Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice. J Biol Chem. 2019;294:13718-13728 pubmed publisher
Tusa I, Gagliardi S, Tubita A, Pandolfi S, Urso C, Borgognoni L, et al. ERK5 is activated by oncogenic BRAF and promotes melanoma growth. Oncogene. 2018;37:2601-2614 pubmed publisher
Durán J, Lagos D, Pávez M, Troncoso M, Ramos S, Barrientos G, et al. Ca2+/Calmodulin-Dependent Protein Kinase II and Androgen Signaling Pathways Modulate MEF2 Activity in Testosterone-Induced Cardiac Myocyte Hypertrophy. Front Pharmacol. 2017;8:604 pubmed publisher
He K, Hu J, Yu H, Wang L, Tang F, Gu J, et al. Serine/Threonine Kinase 40 (Stk40) Functions as a Novel Regulator of Skeletal Muscle Differentiation. J Biol Chem. 2017;292:351-360 pubmed publisher
Gu Z, Churchman M, Roberts K, Li Y, Liu Y, Harvey R, et al. Genomic analyses identify recurrent MEF2D fusions in acute lymphoblastic leukaemia. Nat Commun. 2016;7:13331 pubmed publisher
Trazzi S, Fuchs C, Viggiano R, De Franceschi M, Valli E, Jedynak P, et al. HDAC4: a key factor underlying brain developmental alterations in CDKL5 disorder. Hum Mol Genet. 2016;25:3887-3907 pubmed publisher
Jain P, Lavorgna A, Sehgal M, Gao L, Ginwala R, Sagar D, et al. Myocyte enhancer factor (MEF)-2 plays essential roles in T-cell transformation associated with HTLV-1 infection by stabilizing complex between Tax and CREB. Retrovirology. 2015;12:23 pubmed publisher
product information
Catalog Number :
32967
Product Name :
3XMEF2-luc
article :
doi
id4838
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-promoter modified
backbone_mutation
backbone_originPromega
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
This plasmid was cloned from pFOS WT-GL3 (Addgene #11983), where the human c-fos promoter was removed and replaced with 4 MEF2 sites. See author's map for restriction site/MCS details.
growth strain :
Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3None
cloning_site_5None
promoterMEF2
sequencing_primer_3Luc_N_Rev
sequencing_primer_5RVprimer3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADCAD1, RSRFC4, RSRFC9, mef2
geneMEF2A
id4205
genbank_ids
mutation
nameMEF2 binding sites
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagLuciferase
resistance markers :
399
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA