This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-rNFM
catalog :
32907
citations: 2
Reference
Benito Muñoz M, Matute C, Cavaliere F. Adenosine A1 receptor inhibits postnatal neurogenesis and sustains astrogliogenesis from the subventricular zone. Glia. 2016;64:1465-78 pubmed publisher
Wang L, Ho C, Sun D, Liem R, Brown A. Rapid movement of axonal neurofilaments interrupted by prolonged pauses. Nat Cell Biol. 2000;2:137-41 pubmed
product information
Catalog Number :
32907
Product Name :
pEGFP-rNFM
article :
doi10.1038/35004008
id4819
pubmed_id10707083
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4134
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Plasmid first described in Wang et al. (2000) Rapid movement of axonal neurofilaments interrupted by prolonged pauses Nature Cell Biology 2:137-141.
growth strain :
Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M
origin :
37
pi :
alt_names
NFM
neurofilament protein M
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5ApaI
promoterCMV
sequencing_primer_3multiple primers
sequencing_primer_5multiple primers
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesNef3, Nfm
geneNefm
id24588
genbank_ids
mutation
nameEGFP-rat NFM fusion
shRNA_sequence
size3330
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagEGFP
plasmid copy :
Rat NFM cDNA originally cloned by Ron Liem, Department of Pathology, Columbia University College of Physicians and Surgeons, New York
resistance markers :
1032
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA