This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-hSecurin
catalog :
32903
citations: 1
Reference
Stemmann O, Zou H, Gerber S, Gygi S, Kirschner M. Dual inhibition of sister chromatid separation at metaphase. Cell. 2001;107:715-26 pubmed
product information
Catalog Number :
32903
Product Name :
pCS2-hSecurin
article :
doi10.1016/S0092-8674(01)00603-1
id4859
pubmed_id11747808
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutationModified with N-term ZZ-Tev4 (two IgG binding domains of protein A (ZZ), followed by four TEV-protease cleavage sequences).
backbone_originRZPD
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
in vitro translation
growth notes :
N-terminal tag is two IgG binding domains of protein A (ZZ), followed by four TEV-protease cleavage sequences.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3none
cloning_site_5none
promotersimian CMV IE94
sequencing_primer_3M13 reverse
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEAP1, ECRAR, HPTTG, PTTG, TUTR1
genePTTG1
id9232
genbank_ids
mutation
nameSecurin
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagZZ-Tev4
resistance markers :
256
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA