pcDNA-N1-PDZ2-GAGA | Addgene 32857 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA-N1-PDZ2-GAGA

catalog :

32857

citations: 1

Reference
Weinman E, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S. A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange. Biochemistry. 2003;42:12662-8 pubmed

product information

Catalog Number :

32857

Product Name :

pcDNA-N1-PDZ2-GAGA

article :

doi	10.1021/bi035244l
id	4477
pubmed_id	14580213

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1/hygro(+)

backbone_mutation

none

backbone_origin

Invitrogen

backbone_size

5600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

ERRATUM- Materials & Methods :This in NOT the mouse NHERF-1 cDNA.Paper should read The rabbit cDNA was inserted into. The rabbit cDNA was cut from the pet30a vector & put into pcDNA. The his & S tag comes from the pet30a vector. pcDNA3.1/hygr(+)cut nhe1&xho1 30A_FMZ2.seq cut Xba1 & Xho1 his tag translation = 937

origin :

pi :

alt_names

NHERF

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xho1
cloning_site_5	V=Nhe1 I=Xba1
promoter	cmv
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases
gene	SLC9A3R1
id	100009196

genbank_ids

mutation

PDZ2 P(161)NGYGF mutated to H(161)NGAGA

name

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1

shRNA_sequence

size

1200

species

9986

Oryctolagus cuniculus