This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET30 NHERF1
catalog :
32854
citations: 1
Reference
Weinman E, Steplock D, Tate K, Hall R, Spurney R, Shenolikar S. Structure-function of recombinant Na/H exchanger regulatory factor (NHE-RF). J Clin Invest. 1998;101:2199-206 pubmed
product information
Catalog Number :
32854
Product Name :
pET30 NHERF1
article :
doi10.1172/JCI204
id4807
pubmed_id9593775
bacterial resistance :
Kanamycin
cloning :
backbonepet30a+
backbone_mutationnone
backbone_originNovagen
backbone_size5422
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
pET 30a vector cut with EcoRV NHERF from SK-cut with BSSH II and ECoRI. Both filled in and blunt ligated. insert at bp 5217-6918 aa 1-358 BP#5076 ATG leader protein = 49 +9 from cDNA not usually translated EK cuts 43 AA off sequence nherf tranlates 5244
origin :
37
pi :
alt_names
NHERF
cloning
clone_methodRestriction Enzyme
cloning_site_3V=EcoRV i=EcoR1
cloning_site_5V=EcoRV I =BssH1
promoterT7
sequencing_primer_3T7 terminator primer
sequencing_primer_5T7 promoter primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases
geneSLC9A3R1
id100009196
genbank_ids
U19815
mutationnone
namesolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
shRNA_sequence
size1200
species
9986
Oryctolagus cuniculus
tags
locationN terminal on insert
taghis
locationN terminal on insert
tagS16
resistance markers :
1088
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA