This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ptyrEGFP-TYR
catalog :
32782
citations: 1
Reference
Halaban R, Svedine S, Cheng E, Smicun Y, Aron R, Hebert D. Endoplasmic reticulum retention is a common defect associated with tyrosinase-negative albinism. Proc Natl Acad Sci U S A. 2000;97:5889-94 pubmed
product information
Catalog Number :
32782
Product Name :
ptyrEGFP-TYR
article :
doi10.1073/pnas.97.11.5889
id4778
pubmed_id10823941
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N
backbone_mutationCMV promoter was replaced with 2500bp Tyrosinase promoter from mouse
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
CMV promoter in pEGFP-N vector was replaced with 2500bp of tyrosinase endogenous promoter from mouse. Please note that while the tyrosinase promoter is mouse, the sequence of the tyrosinase insert in this plasmid is human.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3none
cloning_site_5none
promoterTyrosinase
sequencing_primer_3EGFP_N and hTYR-R
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3
geneTYR
id7299
genbank_ids
mutation
nameTyrosinase
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
1082
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA