This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
2-HA-D176A
catalog :
32754
citations: 1
Reference
Nesterov A, Carter R, Sorkina T, Gill G, Sorkin A. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis. EMBO J. 1999;18:2489-99 pubmed
product information
Catalog Number :
32754
Product Name :
2-HA-D176A
article :
doi10.1093/emboj/18.9.2489
id4773
pubmed_id10228163
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237.
origin :
37
pi :
alt_names
2
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAp50, Clapm1, mu2
geneAp2m1
id116563
genbank_ids
mutationD176A
name 2 subunit of clathrin adaptor complex AP-2
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
location
tagHA
resistance markers :
1080
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA