2-HA-WT | Addgene 32752 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

2-HA-WT

catalog :

32752

citations: 2

Reference
Buenaventura T, Bitsi S, Laughlin W, Burgoyne T, Lyu Z, Oqua A, et al. Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells. PLoS Biol. 2019;17:e3000097 pubmed publisher
Nesterov A, Carter R, Sorkina T, Gill G, Sorkin A. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis. EMBO J. 1999;18:2489-99 pubmed

product information

Catalog Number :

32752

Product Name :

2-HA-WT

article :

doi	10.1093/emboj/18.9.2489
id	4773
pubmed_id	10228163

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	ApaI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-Fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Ap50, Clapm1, mu2
gene	Ap2m1
id	116563

genbank_ids

mutation

name

2 subunit of clathrin adaptor complex AP-2

shRNA_sequence

size

species

10116

Rattus norvegicus