This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFR-GFP
catalog :
32751
citations: 50
Reference
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Akhavanfard S, Yehia L, Padmanabhan R, Reynolds J, Ni Y, Eng C. Germline EGFR variants are over-represented in adolescents and young adults (AYA) with adrenocortical carcinoma. Hum Mol Genet. 2021;29:3679-3690 pubmed publisher
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Yao N, Wang C, Liu M, Li Y, Chen W, Li Z, et al. Discovery of a novel EGFR ligand DPBA that degrades EGFR and suppresses EGFR-positive NSCLC growth. Signal Transduct Target Ther. 2020;5:214 pubmed publisher
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Baschieri F, Le Devedec D, Tettarasar S, Elkhatib N, Montagnac G. Frustration of endocytosis potentiates compression-induced receptor signaling. J Cell Sci. 2020;133: pubmed publisher
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Fei Y, Jin Y, Zhao X, Wang Y, Qian J, Su L, et al. The Relative Permittivity Changes of EGF by 50 Hz MF Exposure Neither Affect the Interaction of EGF With EGFR Nor Its Biological Effects. Bioelectromagnetics. 2020;41:241-246 pubmed publisher
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product information
Catalog Number :
32751
Product Name :
EGFR-GFP
article :
doi10.1074/jbc.273.52.35000
id4772
pubmed_id9857032
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4672
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
To create the EGFR-GFP fusion (in-frame), the the full-length cDNA of EGFR including 3' and 5' untranslated regions was cloned into pEGFP-N1 as an XbaI/HindIII fragment. This is EGFR-wt. The 3' end of the EGFR cDNA including STOP codon and untranslated region was removed by PCR and digestion. The 3' primer generated a new SstII (SacII) site.
growth strain :
Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SacII
cloning_site_5XbaI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutation
nameEGFR
shRNA_sequence
size3639
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
plasmid copy :
Ullrich/Joseph Schelessinger, Yale University Axel Ullrich, Max Planck Institute of Biochemistry
resistance markers :
1080
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA