This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)-GRP78/BiP
catalog :
32701
citations: 16
Reference
Allu P, Kiranmayi M, Mukherjee S, Chirasani V, Garg R, Vishnuprabu D, et al. Functional Gly297Ser Variant of the Physiological Dysglycemic Peptide Pancreastatin Is a Novel Risk Factor for Cardiometabolic Disorders. Diabetes. 2022;71:538-553 pubmed publisher
Ei Z, Choochuay K, Tubsuwan A, Pinkaew D, Suksomtip M, Vinayanuwattikun C, et al. GRP78/BiP determines senescence evasion cell fate after cisplatin-based chemotherapy. Sci Rep. 2021;11:22448 pubmed publisher
Saura Esteller J, Sánchez Vera I, Núñez Vázquez S, Cosialls A, Gama Pérez P, Bhosale G, et al. Activation of the Integrated Stress Response and ER Stress Protect from Fluorizoline-Induced Apoptosis in HEK293T and U2OS Cell Lines. Int J Mol Sci. 2021;22: pubmed publisher
Tang X, Luo L, Li Y, Wu H, Hu Q, Yue H, et al. Therapeutic potential of targeting HSPA5 through dual regulation of two candidate prognostic biomarkers ANXA1 and PSAT1 in osteosarcoma. Aging (Albany NY). 2020;13:1212-1235 pubmed publisher
Cicalese S, Okuno K, Elliott K, Kawai T, Scalia R, Rizzo V, et al. 78 kDa Glucose-Regulated Protein Attenuates Protein Aggregation and Monocyte Adhesion Induced by Angiotensin II in Vascular Cells. Int J Mol Sci. 2020;21: pubmed publisher
Machihara K, Namba T. Kuanoniamine C stimulates bortezomib-induced cell death via suppression of glucose-regulated protein 78 in osteosarcoma. Biochem Biophys Res Commun. 2020;527:289-296 pubmed publisher
Sereš M, Pavlíková L, Boháčová V, Kyca T, Borovska I, Lakatos B, et al. Overexpression of GRP78/BiP in P-Glycoprotein-Positive L1210 Cells is Responsible for Altered Response of Cells to Tunicamycin as a Stressor of the Endoplasmic Reticulum. Cells. 2020;9: pubmed publisher
Marcus E, Tokhtaeva E, Jimenez J, Wen Y, Naini B, Heard A, et al. Helicobacter pylori infection impairs chaperone-assisted maturation of Na-K-ATPase in gastric epithelium. Am J Physiol Gastrointest Liver Physiol. 2020;318:G931-G945 pubmed publisher
Shu W, Guo Z, Li L, Xiong Z, Wang Z, Yang Y, et al. Regulation of Molecular Chaperone GRP78 by Hepatitis B Virus: Control of Viral Replication and Cell Survival. Mol Cell Biol. 2020;40: pubmed publisher
Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T. Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78. Biochem Biophys Res Commun. 2017;492:33-40 pubmed publisher
Zhu S, Zhang Q, Sun X, Zeh H, Lotze M, Kang R, et al. HSPA5 Regulates Ferroptotic Cell Death in Cancer Cells. Cancer Res. 2017;77:2064-2077 pubmed publisher
Li J, Huang W, Lin P, Wu B, Fu Z, Shen H, et al. N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma. Sci Rep. 2016;6:35210 pubmed publisher
Thon M, Hosoi T, Ozawa K. Insulin enhanced leptin-induced STAT3 signaling by inducing GRP78. Sci Rep. 2016;6:34312 pubmed publisher
Zanotto Filho A, Masamsetti V, Loranc E, Tonapi S, Gorthi A, Bernard X, et al. Alkylating Agent-Induced NRF2 Blocks Endoplasmic Reticulum Stress-Mediated Apoptosis via Control of Glutathione Pools and Protein Thiol Homeostasis. Mol Cancer Ther. 2016;15:3000-3014 pubmed
Zanotto Filho A, Dashnamoorthy R, Loranc E, de Souza L, Moreira J, Suresh U, et al. Combined Gene Expression and RNAi Screening to Identify Alkylation Damage Survival Pathways from Fly to Human. PLoS ONE. 2016;11:e0153970 pubmed publisher
Werstuck G, Lentz S, Dayal S, Hossain G, Sood S, Shi Y, et al. Homocysteine-induced endoplasmic reticulum stress causes dysregulation of the cholesterol and triglyceride biosynthetic pathways. J Clin Invest. 2001;107:1263-73 pubmed
product information
Catalog Number :
32701
Product Name :
pcDNA3.1(+)-GRP78/BiP
article :
doi10.1172/JCI11596
id4757
pubmed_id11375416
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutationnone
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The D238G point mutation found in the Addgene sequence is a known variation in human GRP78 that does not alter its chaperone or ATPase activities.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XHOI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBIP, GRP78, HEL-S-89n
geneHSPA5
id3309
genbank_ids
mutation
nameGRP78/BiP
shRNA_sequence
size1950
species
9606
Homo sapiens
tags
resistance markers :
1073
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA