This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HSF1-GFPN3
catalog :
32538
citations: 6
Reference
Mota A, Waxman H, Hong R, Lagani G, Niu S, Bertherat F, et al. FOXR1 regulates stress response pathways and is necessary for proper brain development. PLoS Genet. 2021;17:e1009854 pubmed publisher
Ray J, Kruse A, Ozer A, Kajitani T, Johnson R, MacCoss M, et al. RNA aptamer capture of macromolecular complexes for mass spectrometry analysis. Nucleic Acids Res. 2020;48:e90 pubmed publisher
Park H, Yoon N, Lee J, Hu S, Yoon S, Kim S, et al. Unleashing the full potential of Hsp90 inhibitors as cancer therapeutics through simultaneous inactivation of Hsp90, Grp94, and TRAP1. Exp Mol Med. 2020;52:79-91 pubmed publisher
Shin Y, Chang Y, Lee D, Berry J, Sanders D, Ronceray P, et al. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell. 2018;175:1481-1491.e13 pubmed publisher
Purwana I, Liu J, Portha B, Buteau J. HSF1 acetylation decreases its transcriptional activity and enhances glucolipotoxicity-induced apoptosis in rat and human beta cells. Diabetologia. 2017;60:1432-1441 pubmed publisher
Wang X, Grammatikakis N, Siganou A, Calderwood S. Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1. Mol Cell Biol. 2003;23:6013-26 pubmed
product information
Catalog Number :
32538
Product Name :
HSF1-GFPN3
article :
doi10.1128/MCB.23.17.6013-6026.2003
id4715
pubmed_id12917326
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N3
backbone_mutation
backbone_originClontech
backbone_size4729
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationsee depositor comments below
nameHSF1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
1065
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA