This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-HSF1
catalog :
32537
citations: 9
Reference
Huang J, Arii J, Hirai M, Nishimura M, Mori Y. Human herpesvirus 6A nuclear matrix protein U37 interacts with heat shock transcription factor 1 and activates the heat shock response. J Virol. 2023;97:e0071823 pubmed publisher
He L, Lv S, Ma X, Jiang S, Zhou F, Zhang Y, et al. ErbB2 promotes breast cancer metastatic potential via HSF1/LDHA axis-mediated glycolysis. Med Oncol. 2022;39:45 pubmed publisher
Lu W, Omari R, Ray H, Wang J, Williams I, Jacobs C, et al. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. FEBS J. 2022;289:3876-3893 pubmed publisher
Kang H, Oh T, Bahk Y, Kim G, Kan S, Shin D, et al. HSF1 Regulates Mevalonate and Cholesterol Biosynthesis Pathways. Cancers (Basel). 2019;11: pubmed publisher
Carpenter R, Sirkisoon S, Zhu D, Rimkus T, Harrison A, Anderson A, et al. Combined inhibition of AKT and HSF1 suppresses breast cancer stem cells and tumor growth. Oncotarget. 2017;8:73947-73963 pubmed publisher
KrzemieÅ„ Ojak Å, Góral A, Joachimiak E, Filipek A, Fabczak H. Interaction of a Novel Chaperone PhLP2A With the Heat Shock Protein Hsp90. J Cell Biochem. 2017;118:420-429 pubmed publisher
Dayalan Naidu S, Sutherland C, Zhang Y, Risco A, de La Vega L, Caunt C, et al. Heat Shock Factor 1 Is a Substrate for p38 Mitogen-Activated Protein Kinases. Mol Cell Biol. 2016;36:2403-17 pubmed publisher
Carpenter R, Paw I, Dewhirst M, Lo H. Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene. 2015;34:546-57 pubmed publisher
Wang X, Grammatikakis N, Siganou A, Calderwood S. Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1. Mol Cell Biol. 2003;23:6013-26 pubmed
product information
Catalog Number :
32537
Product Name :
FLAG-HSF1
article :
doi10.1128/MCB.23.17.6013-6026.2003
id4715
pubmed_id12917326
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3None
cloning_site_5None
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationsee depositor comments below
nameHSF1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
resistance markers :
1065
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA